Phylogenetics

When to use

Use this workflow for an alignment of homologous loci or proteins, a gene tree, branch support, and an interpretable tree figure. Multi-locus partitioning, concordance factors, ancestral states and dating are covered in IQ-TREE reference.

A gene tree does not establish a species tree, transmission direction, or horizontal transfer by itself. Sampling dates alone do not justify a molecular clock.

Versions and installation

Reviewed against current official documentation on 2026-10-01. The executed smoke workflow used MAFFT 7.526, IQ-TREE 3.1.4, FastTree 2.2.0 and ETE3 3.1.3 on Python 3.12. ETE3 remains deliberate for this helper; ETE4 is a separate API and is not a drop-in replacement. ETE3 imports cgi, removed in Python 3.13. The native executables are not Python packages.

Illustrative installation commands; verify executable versions after installation:

conda create -n phylo -c conda-forge -c bioconda mafft iqtree fasttree trimal
conda activate phylo
uv venv --python 3.12 .venv-phylo
uv pip install --python .venv-phylo/bin/python ete3==3.1.3 numpy six
# Optional rendering backend; it can require a working Qt platform plugin.
uv pip install --python .venv-phylo/bin/python PyQt5

The helper defaults to iqtree3 and FastTree; use --iqtree-bin iqtree2 for an existing IQ-TREE 2 installation, or --fasttree-bin fasttree if that is the installed name. Version 2 compatibility is source-checked, not runtime-tested in this refresh. All analyses are local; this skill invokes no HTTP API or remote inference endpoint.

Workflow

1. Establish the biological input

• Select homologs with appropriate taxon coverage; investigate paralogs, contamination and recombination before assuming one history describes all sites.
• Use unique, whitespace-free FASTA IDs and keep a separate sample metadata table. The helper rejects empty/all-missing records and Newick punctuation in IDs.
• Specify --type nt or --type aa. The helper sends -st DNA or -st AA to IQ-TREE and selects nucleotide/protein FastTree flags explicitly.
• Coding DNA needs codon-aware alignment if using a codon model. Ordinary MAFFT nucleotide alignment does not preserve reading frames automatically.
• Keep the original sequences, accession versions, filters, alignment and exact commands. Inspect alignment lengths, gaps, composition and taxon identities.

2. Align and inspect

These MAFFT commands were exercised on small synthetic FASTA data:

mafft --auto --thread 1 --inputorder sequences.fasta > aligned.fasta
# L-INS-i: locally alignable homologs, typically small datasets.
mafft --localpair --maxiterate 1000 --thread 1 --inputorder sequences.fasta > linsi.fasta
# E-INS-i: homologs with large internal gaps.
mafft --genafpair --maxiterate 1000 --thread 1 --inputorder sequences.fasta > einsi.fasta
# FFT-NS-i (two refinement cycles), then FFT-NS-2 (progressive).
mafft --retree 2 --maxiterate 2 --thread 1 --inputorder sequences.fasta > fftnsi.fasta
mafft --retree 2 --maxiterate 0 --thread 1 --inputorder sequences.fasta > fftns.fasta

auto delegates the choice to MAFFT. Sequence count alone is not an accuracy criterion. Single-thread runs simplify reproducibility; MAFFT iterative refinement with multiple threads can produce different results between runs.

Trimming is optional and must be justified by alignment quality, not assumed to improve every tree. The following trimAl command is documentation-checked and illustrative; trimAl was not installed for this review:

trimal -in aligned.fasta -out trimmed.fasta -automated1 -fasta

Record retained columns/taxa and compare sensitivity to trimming. A failed trim must stop the trimming step; do not silently copy the untrimmed input under a “trimmed” filename. The bundled helper does not trim automatically.

3. Infer a tree

Use the maintained helper instead of recreating subprocess wrappers. From the skill directory, with the executable names on PATH:

python scripts/phylogenetic_analysis.py sequences.fasta --type nt --threads 1 \
  --bootstrap 1000 --seed 42 --output-dir results --no-visualization
# Use a reviewed alignment (including any explicit trimming) without realigning.
python scripts/phylogenetic_analysis.py aligned.fasta --aligned --type nt \
  --threads 1 --output-dir reviewed --no-visualization

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