RELION single-particle refinement

Use for a RELION single-particle project, especially extracted particles → homogeneous selected particle subset → gold-standard refinement → half-map validation and postprocessing. The bundled runner starts from CTF-annotated extracted particles and an initial 3D reference. It does not replace motion correction, picking, 2D/3D selection, or a biological interpretation of map quality. For tomography, helical reconstruction, Blush, or heterogeneous-state modeling, use the appropriate upstream workflow rather than forcing those data into this bounded SPA runner.

Preserve acquisition and coordinate conventions

Read references/acquisition-and-restarts.md when starting from movies or resuming jobs. Confirm pixel size in Å/pixel, voltage in kV, spherical aberration in mm, defocus in Å, amplitude contrast as a fraction, and the symmetry justified by the specimen. Do not “correct” a suspicious value by guessing its units.

data_optics describes acquisition/image groups; data_particles references them through _rlnOpticsGroup. Particle filenames use one-based index@stack.mrcs; leading zeros such as 00000001@stack.mrcs are valid. Relative paths resolve from the RELION project directory, not the STAR file's directory. Keep optics groups when merging or subsetting STAR files. _rlnOriginXAngst/_rlnOriginYAngst are Å translations, not pixels.

Run from this skill directory with paths to the real project:

python scripts/spa_workflow.py validate-star project/particles.star --project project

This opens referenced stacks and checks optics membership, finite acquisition/CTF values, indices, box sizes, duplicate particle references and existing half-set assignments. Use --metadata-only only when stacks are genuinely unavailable; the JSON records stack_checks_performed: false. It does not scan every particle pixel for corruption or establish correct image normalization. Physical-range warnings are review prompts, not proof that unusual microscope settings are wrong.

Refine a selected particle population

Before running, inspect representative particles and class averages, defocus distributions, CTF fits, particle orientation distribution, and the initial reference. Ensure the map and particle boxes/pixel sizes agree after any downsampling. The runner deliberately supports one effective box/pixel size across optics groups; handle heterogeneous sampling with an explicit upstream resampling workflow. Use conventionally extracted, normalized particles that have not already been phase-flipped or Wiener-filtered; this runner does not configure those special input cases.

python scripts/spa_workflow.py refine \
  --star project/particles.star --reference project/initial.mrc \
  --project project --diameter 180 --symmetry C1 \
  --initial-lowpass 40 --mpi-ranks 3 --threads 2 --output project/RefinePilot

The diameter and low-pass filter above are illustrative Å values. Use specimen-appropriate values. Refinement executes mpirun -np 3 relion_refine_mpi with --auto_refine, --split_random_halves, --ctf, and a low-pass starting reference. Gold-standard splitting requires MPI; the plain sequential relion_refine executable cannot perform this split. Use odd ranks ≥3 (master plus balanced half-set workers), with a matching MPI installation. The CPU command is useful for a bounded pilot; choose a documented GPU/MPI launch for full data.

The runner keeps the command, native version and log in a new output directory, records an explicit random seed (default 1), surfaces runtime warnings, stops on process failure, and requires converged unfiltered half maps before reporting success. It does not automatically retry expensive jobs or silently discard failed-job artifacts. Keep _optimiser.star, model/sampling STAR files, and referenced particle paths for restart. Use the original job's optimiser rather than starting a new random split from a partially processed table.

Inspect independent half maps

Use the two independently refined unfiltered half maps, never two copies of the combined, sharpened map. Matching headers cannot establish statistical independence; the independent particle assignments and refinement history provide that evidence. Inspect directional anisotropy, preferred orientation and local resolution as well as a global FSC curve.

…